| ACT 1 | Sung By |
| If You Want to Know Who We Are | Ensemble |
| A Wandering Minstrel | Nanki-Poo, Male Chorus and "Tap-a-Teers" |
| Our Great Mikado | Pish-Tush, Male Chorus and "Tap-a-Teers" |
| Young Man Despair | Pooh-Bah, Pish-Tush and Nanki-Poo |
| Behold the Lord High Executioner | Ko-Ko and Male Chorus |
| I've Got a Little List | Ko-Ko and Male Chorus |
| Comes a Train of Little Ladies | Girls' Chorus |
| Three Little Maids | Yum-Yum, Pitti-Sing, Peep-Bo, "Tap-a-Teers", Dancing Girls and Jitterbugs |
| So Pardon Us | Yum-Yum, Pitti-Sing, Peep-Bo, Pooh-Bah, Pish-Tush and Girls' Chorus |
| Were You Not to Ko-Ko Plighted | Yum-Yum and Nanki-Poo |
| ACT 2 | Sung By |
| Braid the Raven Hair | Ensemble |
| The Moon and I | Yum-Yum and The Harmoneers |
| Here's a How-de-do | Yum-Yum, Nanki-Poo and Ko-Ko |
| The Mikado | The Mikado, Katisha and Entire Ensemble |
| I'm the Emperor of Japan | The Mikado and Katisha |
| My Object All Sublime | The Mikado |
| Flowers That Bloom in the Spring | Pitti-Sing, Yum-Yum, Nanki-Poo, Pooh-Bah and Ko-Ko |
| I, Living I | Katisha |
| Titwillow | Ko-Ko |